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Image Search Results
Journal: Cell chemical biology
Article Title: Morphological subprofile analysis for bioactivity annotation of small molecules.
doi: 10.1016/j.chembiol.2023.06.003
Figure Lengend Snippet: Figure 5. Identification of protein synthesis inhibitors (A) Structures of the ALK2 and ALK3 inhibitor LDN193189 and compound 3. (B) Cluster biosimilarity heatmap for LDN193189 and compound 3. Biosimilarities are given as percentage values. (C) Biosimilarity of LDN193189 and compound 3 to cycloheximide. The top line profile is set as a reference profile (100% biological similarity, BioSim) to which the following profiles are compared. Values were normalized to the DMSO control. Blue color: decreased feature, red color: increased feature. BioSim: biosimilarity, Ind: induction, Conc: concentration. (D) Biosimilarity of LDN193189 and compound 3 the protein synthesis cluster subprofile. (E and F) Influence on protein synthesis in HeLa cells. Cells were treated for 24 h with the compounds or cycloheximide (CHX) and DMSO as controls prior to detection of protein synthesis (D) or cell count (E). Data are mean values (n = 3) ± SD. See also Figure S4 and Table S2.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-alpha-tubulin, FITC-conjugated, mouse monoclonal Sigma Aldrich Cat#F2168; RRID: AB_476967 Chemicals, peptides, and recombinant proteins 40,6-Diamidine-20-phenylindole dihydrochloride (DAPI) Sigma Aldrich Cat#10236276001; CAS: 28718-90-3 DMEM medium (high glucose) PAN Biotech Cat#P04-03550 L-glutamine PAN Biotech Cat#P04-80100 Foetal bovine serum Gibco Cat#10270 Alexa Fluor 594 Phalloidin Thermo Fisher Scientific Cat#A12381; CAS: 330626-83-0 Concanavalin A, Alexa Fluor 488 Conjugate Thermo Fisher Scientific Cat#C11252 Hoechst 33342 Sigma Cat#B2261; CAS: 875756-97-1 MitoTracker Deep Red Thermo Fisher Scientific Cat#M22426; CAS: 873315-86-7 Non-essential amino acids PAN Biotech Cat#P08-32100 Sodium pyruvate PAN Biotech Cat#P04-43100 SYTO 14 Green Fluorescent Nucleic Acid Stain Thermo Fisher Scientific Cat#S7576 Wheat Germ Agglutinin, Alexa Fluor 594 Conjugate Thermo Fisher Scientific Cat#W11262 Critical commercial assays MycoAltertTM Mycoplasma Detection Kit Lonza Cat#LT07-318 HDAC Activity Assay Kit Sigma Aldrich Cat#566328 In SituHDACActivity Fluorometric Assay Kit Sigma Aldrich Cat#EPI003 Click-iT HPG Alexa Fluor 488 Protein Synthesis Assay Kit Thermo Fisher Scientific Inc. Cat#C10428 Deposited data Code to calculate subprofiles and profile biosimilarities This paper https://doi.org/10.5281/zenodo.7969323 Code for calculating profile biosimilarity in the Cell Painting assay (Grigalunas et al., 2021)43 https://doi.org/10.5281/zenodo.4529728 Subprofile explorer Web App This paper https://cpcse.pythonanywhere.com/ Experimental models:
Techniques: Control, Concentration Assay, Cell Counting